Description: Apply now for the Cape Biologix Technologies Bacteria Internship Programme 2021.
As an Intern you will get the opportunity to dive right into an exciting growth phase of one of South Africa’s most promising BioTech startups. You may be an Intern, but your contribution to the team’s success will be significant.
You will support the Production team in our efforts to temporarily increase our capacity to meet worldwide demand for our portfolio of COV-19 proteins. You will be exposed to our innovative plant-based platform as well as learn from our seasoned team of kind but professional production professionals.
The Intern (Bacteria) position will place you in the Bacterial Growth stage of our Upstream process. People interested in the position must have experience in fermentation or large-scale cell culture cultivation.
Location: Ndabeni, Cape Town
Job Description
You will be the right-hand person of the Production Team Leader (Bacteria), providing support in the form of responsibilities such as:
- Conduct growth curves of all bacterial cultures used in production.
- Provide written protocols and reports with conclusions, implications, and recommendations.
- Determine the best harvest point of grown bacterial cultures, (whether it is OD dependent or time dependent).
- Optimise infiltration dilutions and process parameters.
- Conduct stability tests for ready-to-use glycerol stocks and seed stocks.
- Develop and optimise large-scale (13 L) fermentation process for each clone. Assist in infiltration preparations.
- Provide written Process Flows, Batch Books and Reagent Preparation Sheets for upstream production processes.
- Conduct routine upstream microbiological procedures (CFU counts, streak plates, pipetting, serial dilutions).
- Assist Lab Technicians and Senior Scientists when required.
Requirements
- At least an MSC in Microbiology/Chemistry/Biochemistry, but preferably PhDs.
- Experience in fermentation or large-scale cell culture cultivation.
- Experience in bacterial growth optimisation preparation of Master and Working Seed Banks.
- Good scientific writer that has a passion for communicating science in a simple understandable way.
- Own transport as you may be required to work shifts or after hours.
Deliverables
- Development (design, write, edit) of all Bacterial Growth technology transfer documents e.g. SOPs, Flow Diagrams, Batch Books etc.
- - Metric#1: % Documents complete at agreed time.
- Delivery of high standard documents at required formatting and of sound English.
- - Metric #1: Documents require maximum two reviews.
- Master - and Working Seed Banks with required documentation.
- -Metric #1: Seed banks and documentation are delivered at required time and quality.
- Optimisation of Agrobacterium infiltration process by vrowth curve analysis and optimisation of each clone,Optimisation of infiltration process.
- - Metric #1: Repeatable Agrobacterium growth and viability and purity.
Competencies
- Detail Orientation
- Communication
- Analysis/Problem Solving
- Patience
- Self Confidence/Resilience
- Excellent English competency, specifically writing.
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